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Morphological and molecular identification of Habronema spp. (Nematoda: Habronematidae) from donkeys in Xinjiang, China, and notes on the taxonomical status of Habronema majus (Creplin, 1849) and H. microstoma (Schneider, 1866)

发布时间：2017-05-16

Rui Jian . Shi-Wei Wang . Wei-Xin Zhang . Lu-Ping Zhang

Syst Parasitol

DOI 10.1007/s11230-017-9714-8

Abstract

Habronematid nematodes were collected from the stomachs of donkeys, Equus asinus L., in the Tarim Basin, Xinjiang, China. After examination by light and scanning electron microscopy, Habronema muscae
(Carter, 1861) and H. majus (Creplin, 1849) were identified. Themorphology of our specimens representing
H. muscae (Carter, 1861) agreed well with previousredescriptions in the shape of the lateral lips, origin of thelateral alae, ratio of left and right spicules, and number andarrangement of caudal papillae. However, H. maju(Creplin, 1849) differs from H. microstoma (Schneider,1866) in the arrangement of the caudal papillae in the male.Moreover, molecular analysis also showed interspecific differences of 26.2–28.2% in ITS2 and 8.6–8.9% in cox1between H. majus and H. microstoma, a divergence muchhigher than the known intraspecific variation of Habronemaspp. (6.6–8.7% in ITS2; 0.2 2.2% in cox1). The results indicate that both H.microstoma (Schneider, 1866 and H. majus (Creplin, 1849) are valid species.

链接：https://link.springer.com/article/10.1007%2Fs11230-017-9714-8