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Immunopurification and Mass Spectrometry Identifies Protein Phosphatase 2A (PP2A) and BIN2/GSK3 as Regulators of AKS Transcription Factors in Arabidopsis

发布时间：2017-05-03

Shuo-Lei Bu, Chao Liu3 Ning Liu, Ji Long Zhao, Lian Feng Ai, Hao Chi, Kathy L. Li, Chih Wei Chien, Alma L. Burlingame, Sheng-Wei Zhang* and Zhi-Yong Wang*

Molecular Cell

https://doi.org/10.1016/j.molp.2016.09.016

Abstract

ABA induces the phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-responsive kinase substrates; AKS1, AKS2, and AKS3). The unphosphorylated AKSs facilitate stomatal opening through promoting the transcription of genes encoding inwardly rectifying K+channels (Takahashi et al., 2013). AKS1 and AKS3 are also regulators of flowering (Ito et al., 2012). However, the kinases and phosphatases that directly control the phosphorylation status of AKSsin vivohave not been fully characterized. Here, our proteomic analyses provide evidence supporting that AKSs are phosphorylated by GSK3 kinases and dephosphorylated by protein phosphatase 2A (PP2A)

链接：http://www.sciencedirect.com/science/article/pii/S1674205216302295