当前位置：首页>>学术活动 学术活动

学术讲座预告：11月06日 8:30，王鹏程 研究员（中国科学院分子植物卓越中心）：Proteome-Wide Mapping of Protein Kinase Targets in Response to Stress

发布时间：2018-10-30

主  讲  人：王鹏程 研究员

活动时间：11月6日 上午8:30

地      点：理科群3号楼A121室

讲座内容：Proteome-Wide Mapping of Protein Kinase Targets in Response to Stress

Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions, and other features of their target proteins. It is known that protein kinases, such as SNF1-related protein kinases 2 and 3 (SnRK2 and SnRK3), calcium dependent protein kinases (CDPKs), and mitogen activated protein kinases (MAPKs) are central components in plant response to environmental stresses, such as drought, high salinity, cold, and pathogen attack. Although phosphorylation-dependent signaling plays critical roles in plant stress biology, only a small number of targets of these protein kinases have been identified, and consequently how these protein kinases regulate downstream biological processes and mediate stress responses are still largely unknown. In this study, we developed an isotope labeled protein kinase assay-based approach with enriched phosphoproteomes as kinase-substrate pools to identify the putative substrates of eight protein kinases that function in plant abiotic and biotic stress responses. As a result, we identified more than five thousand putative targets from 12 protein kinase-stimuli combinations. We also analyzed the conserved motif and functional ontology of these putative kinase targets. These results provide comprehensive information on the role of these protein kinases in controlling cellular activities and constitute a valuable resource for further studies on the mechanisms underlying plant response to environmental stresses.

主讲人介绍：

王鹏程，2010年河南大学获得植物学博士学位，2011-2017年在美国普渡大学从事博士后研究。在中国科学院分子植物卓越中心/上海植物逆境生物学研究中心担任研究员。主要从事植物蛋白激酶和非生物胁迫信号转导研究。综合利用遗传学、分子生物学和蛋白组学等研究技术解析一些重要蛋白激酶SnRK2、MAPK等及其底物在植物非生物逆境胁迫应答过程中的作用。在植物活性氧信号转导，磷酸化蛋白组学以及植物激素ABA信号转导途径方面取得一系列研究成果。代表性研究成果发表在Molecular Cell、Plant Cell、PNAS、Plant Physiology等国际权威性SCI期刊上。